Description
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Sphingosine (Sph) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Sphingosine (Sph) and unlabeled Sphingosine (Sph) (Standards or samples) with the pre-coated antibody specific to Sphingosine (Sph). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Sphingosine (Sph) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Sphingosine (Sph) in the sample